North Korea has withdrawn from the inter-Korean liaison office which was opened amid a warming of ties last year to facilitate talks with the South.
Seoul said it was contacted on Friday and informed that the North's staff would be leaving later in the day.
It has expressed its regret at the decision and is urging staff from the North to return as soon as possible.
The pullout follows a failed summit between the US and North Korean leaders in Hanoi last month.
The liaison office, located in the North Korean border city of Kaesong, had allowed officials from North and South Korea to communicate on a regular basis for the first time since the Korean War. It is meant to be staffed by up to 20 people from each side.
When the office was opened in September 2018, it was hailed as representing a significant step forward in inter-Korean relations.
The two sides had in the past communicated by fax or special phone lines, which would often be cut when relations soured.
At the time, Seoul's Unification Minister said it would allow for direct discussion of any issues "24 hours, 365 days".
Since last month's failed summit in Vietnam between the US and North Korean leaders, Pyongyang has warned that it could resume missile and nuclear testing.
North Korea Vice Foreign Minister Choe Son-hui said earlier this month that Washington threw away "a golden opportunity" at the summit.
President Trump had said at the time that Mr Kim had asked for the removal of all sanctions - which the US could not agree to. But Ms Choe said that the North had only asked for five key economic sanctions to be lifted.
US officials have insisted that diplomacy is still "alive".